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More recently, increased understanding of nuclease function has led to more direct DNA editing, using techniques such as zinc finger nucleases and CRISPR. The expressed nucleases then knock out and replace genes in the chromosome.As of 2014 these approaches involve removing cells from patients, editing a chromosome and returning the transformed cells to patients.

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The target DNA is then analyzed for the presence of the probe via radioactivity or fluorescence.

In this experiment, as in most molecular biology techniques, a control must be used to ensure successful experimentation.

It must at the same time inquire into genesis and function." Polymerase chain reaction is an extremely versatile technique for copying DNA.